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Image Viewing

Controls for tuning how images display, managing fluorescence channels, navigating Z-stacks and timelapses, and presenting your work. Everything in this panel is non-destructive; the original file is never modified.

Enhance Panel

The Enhance panel adjusts display in real time. Sliders are grouped by category:

Tone

  • Brightness and Contrast for overall exposure
  • Gamma for nonlinear brightness adjustment
  • Highlights and Shadows to target bright and dark areas independently
  • Blackpoint to clip low values to pure black

Color

  • Saturation to adjust color intensity
  • Warmth to shift between cool (blue) and warm (red) tones
  • Tint to shift between green and magenta

Detail

  • Clarity for local contrast enhancement
  • Sharpness for edge enhancement
  • Denoise to reduce noise
  • Deconvolution for optical blur reversal

Visualization

  • Black & White to drop the color channels
  • Invert to flip the tone scale (useful for transmitted light)

Presets and Controls

Pick from built-in presets (high contrast for counting, low noise for smooth appearance, auto-optimize from image statistics) or adjust sliders manually. Use Peek original to see the unenhanced image temporarily, or Reset all to return to defaults.

Channel Controls

For multi-channel fluorescence images, the Channels panel gives you per-channel control:

  • Visibility toggle to show or hide each channel
  • Color assignment with a hex picker per channel
  • Gain slider to adjust per-channel brightness
  • CLAHE (Contrast Limited Adaptive Histogram Equalization) for local contrast enhancement
  • Background subtraction to remove baseline fluorescence

Switch between Composite view (all visible channels blended) and Individual view (each channel in its own grid cell side by side).

Z-Stack and Timelapse

Z-Stack Navigation

For images captured at multiple focal planes:

  • Step through slices with the Z-Stack panel
  • Generate projections along the stack with Maximum, Average, or Sum intensity
  • Open the 3D view for a volumetric render with adjustable opacity and threshold

Timelapse

For time-series acquisitions:

  • Step through frames one at a time
  • Use playback controls with frame-rate display
  • See the current timepoint indicator

Presentation Panel

The Present panel gives you tools for sharing your work:

  • Slideshow to cycle full-screen through images
  • 3D View for the volumetric viewer on Z-stack data
  • Screenshot region to capture an arbitrary region of the current viewport
  • Save image to download the current view with all enhancements applied

Next Steps

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